Dsmz 141

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solution of medium 141, sterilized by filtration. For chemoorganotrophic growth with acetate under air add also 10.00 ml/l of a 5% w/v NaHCO3 solution,.DSMZ culture media database. 141. METHANOGENIUM MEDIUM (H2/CO2). Solution occurs in 192 other media (first defined in medium 141 ).141. METHANOGENIUM MEDIUM (H2/CO2). KCl. 0.34 g. MgCl2 x 6 H2O. 4.00 g. MgSO4 x 7 H2O. 3.45 g. NH4Cl. 0.25 g. CaCl2 x 2 H2O. 0.14 g. K2HPO4. 0.14.Modified Wolins mineral solution (see medium 141). 10.00 ml. Fe(NH4)2(SO4)2 x 6 H2O solution (0.1% w/v). 2.00 ml. Na-acetate. 1.00.Vitamin Solution DSM 141 (see below). 10.00 ml. Fe(NH4)2(SO4)2 x 6 H2O (2% v/v). 1.00 ml. Distilled Water. 1000.00 ml. Trace Element Solution: Na2EDTA.141. METHANOGENIUM MEDIUM (H2/CO2) - DSMZ141a. METHANOCOCCUS SP. MEDIUM - DSMZ1404. MEDIUM FOR STRAIN 2M70-1 - DSMZ

Instructions: Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min.Modified Wolins mineral solution (see medium 141). 10.00 ml. NaHCO3. 2.50 g. Na2-fumarate. 8.00 g. Wolins vitamin solution (see medium 141).(NH4)H2PO4. 2.88 g. MgSO4 x 7 H2O. 0.10 g. Ca(NO3)2 x 4 H2O. 0.05 g. Modified Wolins mineral solution (see medium 141). 10.00 ml. Yeast extract.NH4Cl. 1.00 g. KH2PO4. 0.33 g. K2HPO4. 0.45 g. MgSO4 x 7 H2O. 0.10 g. Modified Wolins mineral solution (see medium 141). 20.00 ml. Yeast extract.For DSM 15288 and DSM 16228 omit Na-acetate and Na-ascorbate. Supplement medium after autoclaving with 10.00 ml/l of a Wolins vitamin solution (see medium 141).133. CARBON MONOXIDE OXIDIZER MEDIUM - DSMZMedium 141 - BacMedia135. ACETOBACTERIUM MEDIUM - DSMZ. juhD453gf

Starch, soluble. 5.0 g. KH2PO4. 0.5 g. Modified Wolins mineral solution (see medium 141). 15.0 ml. NiCl2 x 6 H2O solution (0.1% w/v).Trace Element Solution (see medium 141) (see below). 10.0 ml. Distilled Water. 1000.0 ml. Trace Element Solution. Nitrilotriacetic acid.2009 DSMZ GmbH - All rights reserved. 1024. NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM. Trace solution (see medium 141).Modified Wolins mineral solution (see medium 141). 10.00 ml. Na2SeO4 solution (0.1% w/v). 1.90 ml. NiCl2 x 6 H2O solution (0.1% w/v).Wolins vitamin solution (see medium 141). 10.00 ml. Solution F: Na2S x 9 H2O. 0.40 g. Distilled water. 10.00 ml. Solution A is sparged with 80% N2 and 20%.Solution 3 Trace element solution (medium 141). Nitrilotriacetic acid. 1.50 g. MgSO4 x 7 H2O. 3.00 g. MnSO4 x H2O. 0.50 g. NaCl. 1.00 g. FeSO4 x 7 H2O. 0.10.Wolins vitamin solution (see medium 141). 10.00 ml. L-Cysteine-HCl x H2O. 0.50 g. Na2S x 9 H2O. 0.50 g. Distilled water. 1000.00.Trace element solution(see medium 141). 10.00 ml. Distilled water. 1000.00 ml. Gas with nitrogen gas for about 20 min and then adjust the pH to 6.5 - 6.8.2015 DSMZ GmbH - All rights reserved. Modified Wolins mineral solution (see medium 141). 10.00 ml. Na-DL-lactate. 2.00 g. Yeast extract (OXOID).Modified Wolins mineral solution (see medium 141). 10.00 ml. NH4Cl. 2.70 g. Na-acetate. 2.50 g. Na-resazurin solution (0.1% w/v).Modified Wolins mineral solution (see medium 141). 10.00 ml. Na-formate. 0.20 g. Tryptone (BD Bacto). 1.00 g. Yeast extract (OXOID).Modified Wolins mineral solution (see medium 141). 10.00 ml. Yeast extract (OXOID). 2.00 g. Trypticase peptone (BD BBL).Wolins vitamin solution (see medium 141) sterilized by filtration. Prior to inoculation reduce the complete medium with 10 - 20 mg/l sodium dithionite.Cysteine-HCl x H2O. 0.500 g. Na2S x 9 H2O. 0.500 g. Trace element solution (medium 141). 10.000 ml. Vitamin solution (medium 141). 10.000 ml. Yeast extract.Modified Wolins mineral solution (see medium 141). 10.00 ml. Distilled water. 930.00 ml. Solution B: Na2CO3. 1.50 g. Distilled water.Wolins vitamin solution (see medium 141). 10.00 ml. Na2S x 9 H2O. 0.30 g. L-Cysteine-HCl x H2O. 0.30 g. Distilled water. 1000.00.NH4Cl. 0.50 g. MgSO4 x 7 H2O. 0.16 g. K2HPO4. 1.60 g. Na2HPO4 x H2O. 1.00 g. Modified Wolins mineral solution (see medium 141).Modified Wolins mineral solution (see medium 141). 10.00 ml. (NH4)2Fe(SO4)2 x 6 H2O solution (0.1% w/v). 2.00 ml. (NH4)2Ni(SO4)2 solution (0.1% w/v).Wolins vitamin solution (see medium 141). 10.00 ml. Na2CO3. 1.50 g. Cellobiose. 5.00 g. Na2S x 9 H2O. 0.50 g. Distilled water. 1000.00.Medium 141, anaerobic, 37°C Please follow special instructions: Cultivation of Anaerobes, Cultivation of Methanogens Complete DSMZ Media List.Modified Wolins mineral solution (see medium 141). 10.00 ml. Fe(NH4)2(SO4)2 x 7 H2O solution (0.1% w/v). 2.00 ml. Na-resazurin solution (0.1% w/v).Modified Wolins mineral solution (see medium 141). 10.00 ml. Yeast extract. 10.00 g. Na-resazurin solution (0.1% w/v).Metadata on 141. BRYOCELLA ELONGATA MEDIUM (DSMZ Medium 1286). Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22489.Solution A: NH4Cl. 1.00 g. MgSO4 x 7 H2O. 0.20 g. CaCl2 x 2 H2O. 0.10 g. K2HPO4. 0.05 g. Modified Wolins mineral solution (see medium 141).Modified Wolins mineral solution (see medium 141). 10.00 ml. Na2S2O3 x 5 H2O. 1.00 g. Na-resazurin solution (0.1% w/v). 0.50 ml. Sulfur, powdered.Modified Wolins mineral solution (see medium 141). 10.00 ml. Na-resazurin solution (0.1% w/v). Wolins vitamin solution (see medium 141).Modified Wolins mineral solution (see medium 141). 10.00 ml. Rumen fluid, clarified (see medium 1310). 100.00 ml. Yeast extract.For DSM 22980 after autoclaving add 1 ml of vitamine solution 141 (see Medium141) to. 1 litre of the sterile medium. Incubate in sealed containers.Modified Wolins mineral solution (see medium 141). 10.00 ml. Yeast extract. 0.10 g. Na-acetate. 5.00 g. Na-resazurin solution (0.1% w/v).Modified Wolins mineral solution (see medium 141). 10.00 ml. FeSO4 x 7 H2O solution (0.1% w/v in 0.1 N H2SO4). 2.00 ml. Yeast extract (DIFCO).Modified Wolins mineral solution (see medium 141). 10.00 ml. NaHCO3. 2.50 g. Na-acetate. 3.00 g. Wolins vitamin solution (see medium 141).2014 DSMZ GmbH - All rights reserved. 318. METHANOSARCINA THERMOPHILA (BCYT) MEDIUM. Wolins vitamin solution (see medium 141).Na-resazurin solution (0.1% w/v). 0.50 ml. MOPS (3-(N-morpholino) propane-sulfonic acid). 20.93 g. Wolins vitamin solution (see medium 141). 10.00.Wolins vitamin solution (see medium 141). 10.00 ml. Na2S x 9 H2O. 0.50 g. Distilled water. 1000.00 ml. Dissolve all ingredients except carbonates,.Wolins vitamin solution (see medium 141). 10.00 ml. Distilled water. 1000.00 ml. Dissolve all ingredients except cysteine, carbonate, starch and vitamins.Vitamine Solution 141: Biotin. 2.0 mg. Folic acid. 2.0 mg. Pyridoxine-HCl. 10.0 mg. Thiamine-HCl x 2 H2O. 5.0 mg. Riboflavin. 5.0 mg. Nicotinic acid.Synthetic seawater (2 x conc.) (see below). 500.00 ml. NaHCO3. 0.16 g. KH2PO4. 0.50 g. Modified Wolins mineral solution (see medium 141).@ref: 141 ; BacDive-ID: 7646 ; DSM-Number: 348 ; keywords: Bacteria, genome sequence, mesophilic ; description: Kocuria rhizophila DSM 348 is a mesophilic bacterium.Modified Wolins mineral solution (see medium 141). 10.0 ml. Na-resazurin solution (0.1% w/v). Wolins vitamin solution (see medium 141).

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